The Agar Method: Preparation Continued

  1. While the agar is still hot, immediately centrifuge for 10 minutes at 2000 rpm.

     

  2. Place in the refrigerator until the agar is completely solidified.

     

  1. Gently pry the agar button from the tube with a wooden applicator stick. Do not disturb the cell button at the tip.

     

  2. Using a scalpel blade carefully cut the tip of the agar cone containing the cellular material away from the base of the cone (where there is no cellular material).

 

         

        Figure 11: Cutting the cellular material away from the base of the cone.