The Agar Method: Preparation

  1. After all of the smears/slides have been made, mix up the remaining portion of the sediment by tapping the container gently on the countertop.

     

  2. Melt 4% agar by placing the tube of agar in a small beaker of water and heating it in a microwave for a brief period of time. Do not completely submerge the tube of agar in the water. The level of water in the beaker should be at the same height or slightly higher than the level of the agar in the tube. Make sure the cap of the agar tube is very loose during heating, if it is on too tight pressure will build up inside the tube which may cause it to explode. 

     

  3. Add approximately 4 ml of completely melted agar to the sediment in a centrifuge tube. Mix gently.