Cellular Material Entrapped in Mucus: Preparation Continued

  1. Centrifuge for 10 minutes at 2000 rpm.


  2. Pour off the supernatant fixative.


  3. Place the remaining mass in an appropriately labeled cassette (see Figure 6). If the mass does not hold together well it should be wrapped in lens paper. If it is large, submit a representative portion (there is no diagnostic advantage to submitting multiple cassettes of the same thing).


  4. Place the cassette in fixative and submit for processing as tissue.


             Figure 6: The remaining mass in a cassette.